Expression of the Aminopeptidase N (CD13) Gene in the Human T Cell Lines HuT78 and H9
Identifieur interne : 001305 ( Main/Exploration ); précédent : 001304; suivant : 001306Expression of the Aminopeptidase N (CD13) Gene in the Human T Cell Lines HuT78 and H9
Auteurs : U. Lendeckel [Allemagne] ; Th. Wex [Allemagne] ; Th. K Hne [Allemagne] ; K. Frank [Allemagne] ; D. Reinhold [Allemagne] ; S. Ansorge [Allemagne]Source :
- Cellular Immunology [ 0008-8749 ] ; 1994.
Abstract
Abstract: The membrane-bound metalloprotease aminopeptidase N (APN, CD13, EC 3.4.11.2) is a well-established marker of normal and malignant cells of the myelomonocytic lineages. It is also expressed by leukemic blasts of a small group of patients suffering from acute or chronic lymphoid leukemia. CD13-specific monoclonal antibodies do not bind to the surface of normal B lymphocytes and APN-mRNA was not detectable by Northern analysis in normal lymphocytes or in T cell lines. A recent paper, however, describes the expression of CD13 on concanavalin A-stimulated T cells. Here, by means of enzymatic amplification of cDNA, cloning and sequencing of amplified cDNA fragments, in situ hybridization, and transcription start site mapping, evidence is provided that the human T cell lines HuT78 and H9 do contain APN-mRNA. Ala-pNA-hydrolyzing activity was detected in viable cells and cell homogenates as well as in size-fractionated protein fractions. Alanine-β-naphthylamide (Ala-βNA)-hydrolyzing activity was readily detectable after isoelectric focusing and blotting and was shown to be localized exclusively intracellularly in both cell lines. Usage of aminopeptidase-specific effectors revealed this activity to be distinct from CD13.
Url:
DOI: 10.1006/cimm.1994.1019
Affiliations:
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Ansorge</name><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Research Unit Experimental Immunology, Department of Internal Medicine, University of Magdeburg, Leipziger Strasse 44, D-39120 Magdeburg</wicri:regionArea><placeName><region type="land" nuts="2">Saxe-Anhalt</region><settlement type="city">Magdebourg</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Cellular Immunology</title><title level="j" type="abbrev">YCIMM</title><idno type="ISSN">0008-8749</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1994">1994</date><biblScope unit="volume">153</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="214">214</biblScope><biblScope unit="page" to="226">226</biblScope></imprint><idno type="ISSN">0008-8749</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0008-8749</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The membrane-bound metalloprotease aminopeptidase N (APN, CD13, EC 3.4.11.2) is a well-established marker of normal and malignant cells of the myelomonocytic lineages. It is also expressed by leukemic blasts of a small group of patients suffering from acute or chronic lymphoid leukemia. CD13-specific monoclonal antibodies do not bind to the surface of normal B lymphocytes and APN-mRNA was not detectable by Northern analysis in normal lymphocytes or in T cell lines. A recent paper, however, describes the expression of CD13 on concanavalin A-stimulated T cells. Here, by means of enzymatic amplification of cDNA, cloning and sequencing of amplified cDNA fragments, in situ hybridization, and transcription start site mapping, evidence is provided that the human T cell lines HuT78 and H9 do contain APN-mRNA. Ala-pNA-hydrolyzing activity was detected in viable cells and cell homogenates as well as in size-fractionated protein fractions. Alanine-β-naphthylamide (Ala-βNA)-hydrolyzing activity was readily detectable after isoelectric focusing and blotting and was shown to be localized exclusively intracellularly in both cell lines. Usage of aminopeptidase-specific effectors revealed this activity to be distinct from CD13.</div></front></TEI><affiliations><list><country><li>Allemagne</li></country><region><li>Saxe-Anhalt</li></region><settlement><li>Magdebourg</li></settlement></list><tree><country name="Allemagne"><region name="Saxe-Anhalt"><name sortKey="Lendeckel, U" sort="Lendeckel, U" uniqKey="Lendeckel U" first="U." last="Lendeckel">U. Lendeckel</name></region><name sortKey="Ansorge, S" sort="Ansorge, S" uniqKey="Ansorge S" first="S." last="Ansorge">S. Ansorge</name><name sortKey="Frank, K" sort="Frank, K" uniqKey="Frank K" first="K." last="Frank">K. Frank</name><name sortKey="K Hne, Th" sort="K Hne, Th" uniqKey="K Hne T" first="Th." last="K Hne">Th. K Hne</name><name sortKey="Reinhold, D" sort="Reinhold, D" uniqKey="Reinhold D" first="D." last="Reinhold">D. Reinhold</name><name sortKey="Wex, Th" sort="Wex, Th" uniqKey="Wex T" first="Th." last="Wex">Th. Wex</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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